Journal: Light, Science & Applications

Article Title: From genotype to phenotype: decoding mutations in blasts by holo-tomographic flow cytometry

doi: 10.1038/s41377-025-01913-y

Figure Lengend Snippet: Label-free technique for recognizing NPM1 mutations in AML. a Sketch of the HTFC recording system. PBS polarizing beam splitter, M mirror, MO microscope objective, MC microfluidic channel, BS beam splitter, CMOS camera. b The 5120 × 5120 digital hologram taken from the recorded HTFC video sequence, with a highlighted in red a 384 × 384 holographic ROI. According to the reference system, cells flow along the y -axis and rotate around the x -axis

Article Snippet: The object beam illuminates the microfluidic channel (Microfluidic ChipShop 10000107 − L z = 200 μm, L x = 1000 μm, L y = 58.5 mm) while cells are flowing along the y -axis and rotating around the x -axis thanks to the hydrodynamic forces of a laminar flow generated at ~50 nL s −1 by an automatic syringe pump (CETONI Syringe Pump neMESYS 290N).

Techniques: Microscopy, Sequencing

Journal: Disease Models & Mechanisms

Article Title: Modeling ANKRD26 5′-UTR mutation-related thrombocytopenia

doi: 10.1242/dmm.052222

Figure Lengend Snippet: Thrombocyte counts and their adhesion/aggregation on a collagen surface under flow. (A-C) Total (A), young (B) and mature (C) thrombocyte counts in wt ( n =12), ankrd26 ku6/+ ( n =15) and ankrd26 ku6 ( n =12) zebrafish. The data shown represent the individual values, mean and s.e.m. Kruskal–Wallis analysis was used to determine statistical significance. (D) The surface coverage of fluorescent thrombocytes on a fibrillar collagen-coated surface in the microfluidic channel after perfusion of pooled whole blood obtained from wt (top) and ankrd26 ku6 (bottom) zebrafish under arterial shear (15 dyne/cm 2 ). (E) The rate of fluorescence accumulation (or thrombocyte adhesion) on a fibrillar collagen-coated surface following perfusion of pooled whole blood from wt and ankrd26 ku6 zebrafish. Data are presented as the mean±s.e.m. from three independent experiments. ns, P >0.05; * P <0.05 and *** P <0.005.

Article Snippet: PPACK-anticoagulated whole-blood samples pooled from adult zebrafish (6-8 months old, n =10 each group) of different genotypes were perfused at 15 dyne/cm2 over type I fibrillar collagen-coated surfaces using parallel microfluidic channels in the BioFlux system (Fluxion Biosciences, Oakland, CA, USA).

Techniques: Shear, Fluorescence